Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Monkey |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE33-17 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 41 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human HSC70 Interacting Protein HIP aa 270-369 / 369. |
Positive control: | A431 cell lysate, HeLa cell lysate, A549 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P50502 Human |
Alternative names: | AAG 2 AAG2 Aging associated protein 2 F10A1_HUMAN FAM10A1 FAM10A4 FLJ27260 Heat shock 70kD protein binding protein Hip HOP Hsc70 interacting protein Hsc70-interacting protein Hsp70 interacting protein HSPABP 1 HSPABP HSPABP1 MGC129952 OTTHUMP00000028873 P48 PRO0786 Progesterone receptor associated p48 protein Progesterone receptor-associated p48 protein Protein FAM10A1 Putative tumor suppressor ST13 Renal carcinoma antigen NY REN 33 Renal carcinoma antigen NY-REN-33 SNC 6 SNC6 ST 13 St13 Suppression of tumorigenicity 13 Suppression of tumorigenicity 13 (colon carcinoma) Suppression of tumorigenicity 13 protein Suppression of tumorigenicity protein 13 |
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Fig1:
Western blot analysis of HSC70 Interacting Protein HIP on different lysates with Rabbit anti-HSC70 Interacting Protein HIP antibody (HA722274) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: HeLa cell lysate Lane 3: A549 cell lysate Lane 4: COS-1 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |