NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45]
cat.: HA722283
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH05-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NCOA4 aa 1-400 / 614. |
| Positive control: | Human kidney tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:500-1:1,500 1:200 |
| Uniprot #: | SwissProt: Q13772 Human Entrez Gene: 27057 Mouse | 619385 Rat |
| Alternative names: | 70 kDa androgen receptor activator 70 kDa androgen receptor coactivator 70 kDa AR activator 70 kDa AR-activator Androgen receptor coactivator 70 kD Androgen receptor coactivator 70 kDa protein Androgen receptor-associated protein of 70 kDa ARA 70 ARA70 DKFZp762E1112 ELE 1 ELE1 ELE1/ret TK NCOA 4 NCoA-4 NCOA4 NCOA4_HUMAN Nuclear receptor coactivator 4 Papillary thyroid carcinoma 3 PTC 3 PTC3 RET activating gene ELE1 Ret activating protein ELE1 Ret-activating protein ELE1 RFG |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.