CA12 Recombinant Rabbit Monoclonal Antibody [PSH05-49]
cat.: HA722287
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH05-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CA12 aa 1-301 / 354. |
| Positive control: | A549 cell lysate, MCF7 cell lysate, A549. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: O43570 Human |
| Alternative names: | CA 12 CA XII CA-XII CA12 CAH12_HUMAN Carbonate dehydratase XII Carbonic anhydrase 12 Carbonic anhydrase XII Carbonic dehydratase CAXII FLJ20151 HsT18816 T18816 Tumor antigen HOM RCC 3.1.3 Tumor antigen HOM-RCC-3.1.3 |
Images
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Fig1:
Western blot analysis of CA12 on different lysates with Rabbit anti-CA12 antibody (HA722287) at 1/2,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Predicted band size: 40 kDa Observed band size: 45 kDa Exposure time: 54 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722287) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A549 cells labeling CA12 with Rabbit anti-CA12 antibody (HA722287) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CA12 antibody (HA722287) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of A549 cells labeling CA12. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722287, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.