Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50]
cat.: HA722288
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH05-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Tau aa 571-620 / 758. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, rat kidney tissue, rat colon tissue. |
| Subcellular location: | Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:1,000 1:5,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P10637 Mouse | P19332 Rat |
| Alternative names: | AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU TAU_HUMAN Tauopathy and respiratory failure Tauopathy and respiratory failure, included |
Images
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Fig1:
Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (hot lysis) Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (hot lysis) Lane 2: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 50 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Tau was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722288 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722288 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA722288 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA722288 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 50 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.