Niemann Pick C2 Recombinant Rabbit Monoclonal Antibody [PSH05-52]
cat.: HA722294
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH05-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Niemann Pick C2 aa 1-151 / 151. |
| Positive control: | HepG2 cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Secreted, Endoplasmic reticulum, Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:100,000-1:300,000 |
| Uniprot #: | SwissProt: P61916 Human | Q9Z0J0 Mouse Entrez Gene: 286898 Rat |
| Alternative names: | EDDM1 Epididymal protein 1 Epididymal secretory protein Epididymal secretory protein E1 hE1 Human epididymis-specific protein 1 Niemann-Pick disease type C2 Niemann-Pick disease type C2 protein NPC intracellular cholesterol transporter 2 NPC2 NPC2_HUMAN Tissue specific secretory protein |
Images
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Fig1:
Western blot analysis of Niemann Pick C2 on different lysates with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: RAW264.7 cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: C6 cell lysate (10 µg/Lane) Lane 6: Mouse kidney tissue lysate (20 µg/Lane) Lane 7: Mouse spleen tissue lysate (20 µg/Lane) Lane 8: Rat kidney tissue lysate (20 µg/Lane) Lane 9: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 16 kDa Observed band size: 16-18 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722294) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/100,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/100,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/300,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/300,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.