HMGA2 Recombinant Rabbit Monoclonal Antibody [PSH05-53]
cat.: HA722295
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH05-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HMGA2 aa 70-120. |
| Positive control: | HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, NIH/3T3, human colon cancer tissue, mouse colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC IF-Tissue |
1:1,000 1:1,000 1:8,000 1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P52926 Human | P52927 Mouse |
| Alternative names: | HMGA 2 BABL High mobility group (nonhistone chromosomal) protein isoform I C High mobility group (nonhistone chromosomal) protein isoform IC High Mobility Group AT hook 2 High Mobility Group AT hook protein 2 High mobility group AT-hook protein 2 High mobility group protein HMGI C High mobility group protein HMGI-C High mobility group protein HMGIC High mobility group protein I, isoform C HMGA2 HMGA2_HUMAN HMGI C HMGIC LIPO Non histone chromosomal architectural protein HMGI C Non histone chromosomal architectural protein HMGIC Pygmy STQTL9 |
Images
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Fig1:
Western blot analysis of HMGA2 on different lysates with Rabbit anti-HMGA2 antibody (HA722295) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 18 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722295) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling HMGA2 with Rabbit anti-HMGA2 antibody (HA722295) at 1/2,500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HMGA2 antibody (HA722295) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-HMGA2 antibody (HA722295) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722295) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-HMGA2 antibody (HA722295) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722295) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of NIH/3T3 cells labeling HMGA2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722295, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.