CREBBP Recombinant Rabbit Monoclonal Antibody [PS01-16]
cat.: HA722297
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PS01-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 265 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CREBBP aa 2400-2500. |
| Positive control: | Human colon carcinoma tissue, human breast carcinoma tissue, mousse testis tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
IHC-P |
1:200-1:1,000 |
| Uniprot #: | SwissProt: Q92793 Human | P45481 Mouse |
| Alternative names: | CBP CBP_HUMAN CREB binding protein CREB-binding protein Crebbp Cyclic AMP responsive enhancer binding protein KAT3A RSTS RTS Rubinstein Taybi syndrome |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CREBBP antibody (HA722297) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722297) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-CREBBP antibody (HA722297) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722297) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mousse testis tissue with Rabbit anti-CREBBP antibody (HA722297) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722297) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.