TLR9 Recombinant Rabbit Monoclonal Antibody [JE59-91]
cat.: HA722299
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE59-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 116 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TLR9 aa 361-560 / 1,032. |
| Positive control: | Daudi cell lysate, Raji cell lysate, RPMI 8226 cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle, phagosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9NR96 Human |
| Alternative names: | CD 289 CD289 TLR 9 TLR9 TLR9_HUMAN Toll like receptor 9 Toll like receptor 9 isoform A precursor Toll like receptor 9 isoform B Toll-like receptor 9 |
Images
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Fig1:
Western blot analysis of TLR9 on different lysates with Rabbit anti-TLR9 antibody (HA722299) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lane 3: THP-1 cell lysate (negative) Lane 4: RPMI 8226 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 116 kDa Observed band size: 116 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722299) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.