Inhibin alpha Recombinant Rabbit Monoclonal Antibody [PD01-29]
cat.: HA722301
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PD01-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Inhibin alpha aa 233-366. |
| Positive control: | Mouse ovary tissue lysate, mouse testis tissue lysate, rat ovary tissue lysate, rat testis tissue lysate, human adrenal gland tissue, human ovary granular cell tumor tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P WB |
1:500-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P05111 Human | Q04997 Mouse | P17490 Rat |
| Alternative names: | A inhibin subunit A inhibin subunit precursor AW55578 IHA inhA INHA_HUMAN Inhibin alpha chain Inhibin alpha chain precursor Inhibin alpha subunit |
Images
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Fig1:
Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody (HA722301) at 1/1,000 dilution. Lane 1: Mouse ovary tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat ovary tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40-45 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722301) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-Inhibin alpha antibody (HA722301) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722301) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human ovary granular cell tumor tissue with Rabbit anti-Inhibin alpha antibody (HA722301) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722301) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.