TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH05-56]
cat.: HA722303
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH05-56
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 30 kDa
Isotype: IgG
Immunogen: Recombinant protein within human TNFRSF14 aa 1-223 / 283.
Positive control: SK-MEL-28 cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q92956 Human
Alternative names: HVEML ATAR CD270 CD40 like protein precursor Herpes virus entry mediator A Herpesvirus entry mediator A Herpesvirus entry mediator Herpesvirus entry mediator ligand HveA HVEM HVEM L LIGHT LIGHTR TNFRSF14 TNFSF 14 TNR14_HUMAN TR2 Tumor necrosis factor receptor like gene2 Tumor necrosis factor receptor superfamily member 14 Tumor necrosis factor receptor superfamily member 14 precursor Tumor necrosis factor receptor-like 2
Images
HA722303_1.jpg Fig1: Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA722303) at 1/1,000 dilution.

Lane 1: SK-MEL-28 cell lysate
Lane 2: SK-MEL-28 cell lysate treated with deglycosylation
Lane 3: A549 cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 30 kDa
Observed band size: 40-50 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722303) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.