HOXA13 Recombinant Rabbit Monoclonal Antibody [JE33-37]
cat.: HA722305
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE33-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HOXA13 aa 250-350. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse liver tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P31271 Human | Q62424 Mouse Entrez Gene: 500129 Rat |
| Alternative names: | Homeo box 1J Homeo box A13 Homeobox 1J Homeobox A13 Homeobox protein Hox A13 Homeobox protein Hox-1J Homeobox protein Hox-A13 Homeobox protein HOXA13 Homeobox1J HomeoboxA13 HOX 1 HOX 1J Hox 1J protein HOX A13 HOX1 HOX1J HOXA 13 HOXA13 HXA13_HUMAN Transcription factor HOXA13 |
Images
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Fig1:
Western blot analysis of HOXA13 on different lysates with Rabbit anti-HOXA13 antibody (HA722305) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 49 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HOXA13 antibody (HA722305) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722305) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.