CRYAA Recombinant Rabbit Monoclonal Antibody [JE35-23]
cat.: HA722306
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CRYAA aa 1-100 / 173. |
| Positive control: | Mouse eyeball tissue lysate, rat eyeball tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P24622 Mouse | P24623 Rat |
| Alternative names: | Acry 1 Alpha crystallin A chain Alpha-crystallin A chain CRYA 1 CRYA1 CRYAA CRYAA_HUMAN Crystallin alpha 1 Crystallin alpha A Heat shock protein beta 4 Heat shock protein beta-4 HSPB 4 HspB4 short form Zonular Central Nuclear Cataract |
Images
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Fig1:
Western blot analysis of CRYAA on different lysates with Rabbit anti-CRYAA antibody (HA722306) at 1/1,000 dilution. Lane 1: Mouse eyeball tissue lysate Lane 2: Rat eyeball tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.