Retinoic Acid Receptor beta Recombinant Rabbit Monoclonal Antibody [JE36-74]
cat.: HA722308
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Retinoic Acid Receptor beta aa 356-455 / 455. |
| Positive control: | U-87 MG cell lysate, MCF7 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P10826 Human |
| Alternative names: | HAP HBV-activated protein NR1B2 Nuclear receptor subfamily 1 group B member 2 RAR B RAR beta RAR epsilon RAR-beta RAR-epsilon RARB RARB_HUMAN Retinoic acid receptor beta 2 Retinoic acid receptor beta 4 Retinoic acid receptor beta 5 Retinoic acid receptor beta Retinoic acid receptor beta polypeptide RRB2 |
Images
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Fig1:
Western blot analysis of Retinoic Acid Receptor beta on different lysates with Rabbit anti-Retinoic Acid Receptor beta antibody (HA722308) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722308) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.