GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09]
cat.: HA722314
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE41-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GUCY1B3 aa 1-100 / 619. |
| Positive control: | Human lung tissue lysate, human brain tissue lysate, mouse lung tissue lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, rat spleen tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q02153 Human | O54865 Mouse | P20595 Rat |
| Alternative names: | GC-S-beta-1 GC-SB3 GCbeta1 GCS beta 1 GCS beta 3 GCS-beta-1 GCS-beta-3 GCYB1_HUMAN guanylate cyclase 1, soluble, beta 3 Guanylate cyclase soluble beta 1 chain Guanylate cyclase soluble subunit beta-1 Guanylate cyclase soluble subunit beta-3 GUC1B3 GUCSB3 GUCY1B1 Gucy1b1 GUCY1B3 SGC Soluble guanylate cyclase small subunit |
Images
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Fig1:
Western blot analysis of GUCY1B3 on different lysates with Rabbit anti-GUCY1B3 antibody (HA722314) at 1/1,000 dilution. Lane 1: Human lung tissue lysate Lane 2: Human brain tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722314) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.