Phospho-LRRK2 (S935) Recombinant Rabbit Monoclonal Antibody [JE42-11]
cat.: HA722315
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE42-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 286 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser935 of human LRRK2 protein. |
| Positive control: | MEF treated with 1μM okadaic acid for 1 hour cell lysate, MEF treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, MEF cells treated with 100nM Calyculin A for 30 minutes. |
| Subcellular location: | Cytoplasmic vesicle, Perikaryon, Golgi apparatus membrane, Cell projection, axon, dendrite, Endoplasmic reticulum membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Endosome, Lysosome, Mitochondrion outer membrane, Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q5S007 Human | Q5S006 Mouse |
| Alternative names: | augmented in rheumatoid arthritis 17 AURA17 Dardarin Leucine rich repeat kinase 2 leucine rich repeat serine threonine protein kinase 2 Leucine-rich repeat serine/threonine-protein kinase 2 LRRK 2 LRRK2 LRRK2_HUMAN PARK 8 PARK8 RIPK7 ROCO 2 ROCO2 |
Images
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Fig1:
Western blot analysis of Phospho-LRRK2 (S935) on different lysates with Rabbit anti-Phospho-LRRK2 (S935) antibody (HA722315) at 1/1,000 dilution. Lane 1: MEF cell lysate (20 µg/Lane) Lane 2: MEF treated with 1μM okadaic acid for 1 hour cell lysate (20 µg/Lane) Lane 3: MEF cell lysate (20 µg/Lane) Lane 4: MEF treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (10 µg/Lane) Lane 6: MEF treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour (20 µg/Lane) Predicted band size: 286 kDa Observed band size: 286 kDa Exposure time: 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722315) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MEF cells treated with 100nM Calyculin A for 30 minutes labeling Phospho-LRRK2 (S935) with Rabbit anti-Phospho-LRRK2 (S935) antibody (HA722315) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LRRK2 (S935) antibody (HA722315) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of MEF cells (left) and MEF cells treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-LRRK2 (S935). Cells were fixed and permeabilized. Then stained with the primary antibody (HA722315, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.