Phospho-Beta Catenin (T41/S45) Recombinant Rabbit Monoclonal Antibody [JE54-02]
cat.: HA722316
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr41/Ser45 of human Beta catenin. |
| Positive control: | HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P35222 Human | Q02248 Mouse | Q9WU82 Rat |
| Alternative names: | b-catenin Beta catenin Beta-catenin Cadherin associated protein Catenin (cadherin associated protein), beta 1, 88kDa Catenin beta 1 Catenin beta-1 CATNB CHBCAT CTNB1_HUMAN CTNNB CTNNB1 DKFZp686D02253 FLJ25606 FLJ37923 OTTHUMP00000162082 OTTHUMP00000165222 OTTHUMP00000165223 OTTHUMP00000209288 OTTHUMP00000209289 |
Images
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Fig1:
Western blot analysis of Phospho-Beta Catenin (T41/S45) on different lysates with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lane 7: C6 cell lysate Lane 8: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: C6 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 30 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-9: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722316) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.