Rad51D Recombinant Rabbit Monoclonal Antibody [JE63-20]
cat.: HA722319
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Rad51D aa 1-150 / 328. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, ES-W4 cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome, telomere. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O75771 Human | O55230 Mouse Entrez Gene: 303375 Rat |
| Alternative names: | BROVCA4 DNA repair protein RAD51 homolog 4 HsTRAD OTTHUMP00000163851 OTTHUMP00000163852 OTTHUMP00000163853 R51H3 RA51D_HUMAN RAD51 homolog D (S. cerevisiae) RAD51 homolog D RAD51 like 3 (S. cerevisiae) RAD51 paralog D RAD51, S. cerevisiae, homolog of, D RAD51-like protein 3 Rad51l3 Recombination repair protein S. cerevisiae RAD51-like 3 TRAD |
Images
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Fig1:
Western blot analysis of Rad51D on different lysates with Rabbit anti-Rad51D antibody (HA722319) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: ES-W4 cell lysate Lane 5: C6 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722319) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.