NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56]
cat.: HA722320
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE77-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NELF-B aa 1-250 / 580. |
| Positive control: | T-47D cell lysate, HeLa cell lysate, BxPC-3 cell lysate, HepG2 cell lysate, MCF7 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, L6 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8WX92 Human | Q8C4Y3 Mouse Entrez Gene: 311796 Rat |
| Alternative names: | A730008L03Rik AB041607 AI663983 Cobra1 Cofactor of BRCA1 DKFZp586B0519 KIAA1182 Negative elongation factor B Negative elongation factor protein B Nelf b NELF-B NELFB RGD1307832 RP13-122B23.3 RP23-132N23.4 |
Images
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Fig1:
Western blot analysis of NELF-B on different lysates with Rabbit anti-NELF-B antibody (HA722320) at 1/1,000 dilution. Lane 1: T-47D cell lysate Lane 2: HeLa cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: C2C12 cell lysate Lane 10: C6 cell lysate Lane 11: L6 cell lysate Lane 12: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: Lane 1-7: 2 minutes; Lane 8-12: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722320) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.