PSAP Recombinant Rabbit Monoclonal Antibody [JE33-54]
cat.: HA722322
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PSAP aa 1-150 / 524. |
| Positive control: | HepG2 cell lysate, U-2 OS cell lysate, U-87 MG cell lysate, A549 cell lysate, SK-Br-3 cell lysate, LNCaP cell lysate, MCF7 cell lysate. |
| Subcellular location: | Lysosome; Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P07602 Human |
| Alternative names: | A1 activator Cerebroside sulfate activator Co-beta-glucosidase Component C CSAct Dispersin GLBA Glucosylceramidase activator Proactivator polypeptide Proactivator polypeptide precursor Prosaposin (sphingolipid activator protein 1) prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) Prosaposin Protein A Protein C PSAP SAP 1 SAP 2 SAP-1 SAP-2 SAP_HUMAN SAP1 Saposin A Saposin B Saposin B Val Saposin C Saposin D Saposin-D Saposins Sgp1 Sphingolipid activator protein 1 Sphingolipid activator protein 2 Sulfated glycoprotein 1 Sulfatide/GM1 activator |
Images
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Fig1:
Western blot analysis of PSAP on different lysates with Rabbit anti-PSAP antibody (HA722322) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: U-2 OS cell lysate Lane 3: U-87 MG cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: LNCaP cell lysate Lane 7: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 80 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722322) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.