PDE4B Recombinant Rabbit Monoclonal Antibody [JE33-95]
cat.: HA722323
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE33-95 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment within Human PDE4B aa 550 to the C-terminus. |
| Positive control: | A549 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, mouse brain tissue, A549. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q07343 Human Entrez Gene: 18578 Mouse |
| Alternative names: | 5'-cyclic phosphodiesterase 4B cAMP-specific 3' cAMP-specific 3',5'-cyclic phosphodiesterase 4B cAMP-specific phosphodiesterase-4 B isoform DKFZp686F2182 DPDE4 dunce-like phosphodiesterase E4 MGC126529 OTTHUMP00000010652 OTTHUMP00000010653 OTTHUMP00000010654 OTTHUMP00000010656 OTTHUMP00000232365 PDE 4B PDE IVB PDE32 Pde4b PDE4B_HUMAN PDE4B5 PDEIVB Phosphodiesterase 4B Phosphodiesterase 4B cAMP specific Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila) |
Images
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Fig1:
Western blot analysis of PDE4B on different lysates with Rabbit anti-PDE4B antibody (HA722323) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Mouse heart tissue lysate (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE4B antibody (HA722323) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722323) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of A549 cells labeling PDE4B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722323, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.