KLF10 Recombinant Rabbit Monoclonal Antibody [JE34-09]
cat.: HA722324
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE34-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human KLF10 aa 1-100 / 480. |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, K-562 cell lysate, mouse lung tissue lysate, mouse testis tissue lysate, rat thymus tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13118 Human | O89091 Mouse | O08876 Rat |
| Alternative names: | Early growth response, alpha Early growth response, alpha like EGR A EGR alpha EGR(A) EGR-alpha EGRA Egral EGRalpha Gdnfif KLF 10 KLF10 KLF10_HUMAN Krueppel like factor 10 Krueppel like factor10 Krueppel-like factor 10 Kruppel like factor 10 mGIF TGF-beta- inducible early gene TGFB inducible early growth response 1 TGFB inducible early growth response TGFB inducible early growth response protein 1 TGFB-inducible early growth response protein 1 TIEG 1 TIEG TIEG-1 TIEG1 Transforming growth factor beta inducible early growth response Transforming growth factor beta inducible early growth response protein 1 Transforming growth factor-beta-inducible early growth response protein 1 Zinc finger transcription factor TIEG |
Images
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Fig1:
Western blot analysis of KLF10 on different lysates with Rabbit anti-KLF10 antibody (HA722324) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 53 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.