VPS18 Recombinant Rabbit Monoclonal Antibody [JE34-83]
cat.: HA722325
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE34-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human VPS18 aa 1-100. |
| Positive control: | HeLa cell lysate, A549 cell lysate, K-562 cell lysate, rat testis tissue. |
| Subcellular location: | Late endosome membrane, Lysosome membrane, Early endosome, Cytoplasmic vesicle, autophagosome, clathrin-coated vesicle. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q9P253 Human Entrez Gene: 296083 Rat |
| Alternative names: | hVPS18 PEP3 Vacuolar protein sorting 18 Vacuolar protein sorting protein 18 Vacuolar protein sorting-associated protein 18 homolog vps18 VPS18_HUMAN |
Images
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Fig1:
Western blot analysis of VPS18 on different lysates with Rabbit anti-VPS18 antibody (HA722325) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722325) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-VPS18 antibody (HA722325) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722325) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.