Neuronatin Recombinant Rabbit Monoclonal Antibody [JE35-00]
cat.: HA722326
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE35-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 9 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Neuronatin aa 32-81 / 81. |
| Positive control: | SH-SY5Y cell lysate, E14.0 mouse brain tissue lysate, mouse embryo tissue lysate, rat embryo tissue lysate, human brain tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q16517 Human | Q61979 Mouse | Q62649 Rat |
| Alternative names: | Neuronatin NNAT NNAT_HUMAN Peg 5 Peg5 |
Images
|
Fig1:
Western blot analysis of Neuronatin on different lysates with Rabbit anti-Neuronatin antibody (HA722326) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SK-Br-3 cell lysate (negative) Lane 3: E14.0 mouse brain tissue lysate Lane 4: Mouse embryo tissue lysate Lane 5: Rat embryo tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 9 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722326) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Neuronatin antibody (HA722326) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722326) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.