Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE35-40 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 114 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Mov10 aa 841-890 / 1,003. |
Positive control: | HEK-293 cell lysate, HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, rat liver tissue lysate, rat kidney tissue lysate. |
Subcellular location: | Cytoplasm, P-body, Cytoplasmic ribonucleoprotein granule, Stress granule, Nucleus. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q9HCE1 Human | P23249 Mouse Entrez Gene: 310756 Rat |
Alternative names: | DKFZp667O1423 FLJ32791 fSAP113 Functional spliceosome-associated protein 113 gb110 MGC2948 Moloney leukemia virus 10 protein MOV 10 Mov10 (Moloney leukemia virus 10, mouse) homolog Mov10 (Moloney leukemia virus 10,) homolog Mov10, Moloney leukemia virus 10, homolog (mouse) Mov10, Moloney leukemia virus 10, homolog Putative helicase MOV10 |
Fig1:
Western blot analysis of Mov10 on different lysates with Rabbit anti-Mov10 antibody (HA722328) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: C6 cell lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 114 kDa Observed band size: 114 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722328) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |