| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE37-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Apo-M aa 1-100 / 188. |
| Positive control: | Human plasma lysate, Mouse plasma lysate, Rat plasma lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95445 Human | Q9Z1R3 Mouse | P14630 Rat |
| Alternative names: | Apo M Apo-M Apolipoprotein M ApoM APOM_HUMAN G3A HSPC336 MGC22400 NG20 NG20 like protein Protein G3a |
|
Fig1:
Western blot analysis of Apo-M on different lysates with Rabbit anti-Apo-M antibody (HA722333) at 1/1,000 dilution. Lane 1: Human plasma lysate Lane 2: Mouse plasma lysate Lane 3: Rat plasma lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 21 kDa Observed band size: 21/25 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722333) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |