Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE37-03 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 21 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Apo-M aa 1-100 / 188. |
Positive control: | Human plasma lysate, Mouse plasma lysate, Rat plasma lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: O95445 Human | Q9Z1R3 Mouse | P14630 Rat |
Alternative names: | Apo M Apo-M Apolipoprotein M ApoM APOM_HUMAN G3A HSPC336 MGC22400 NG20 NG20 like protein Protein G3a |
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Fig1:
Western blot analysis of Apo-M on different lysates with Rabbit anti-Apo-M antibody (HA722333) at 1/1,000 dilution. Lane 1: Human plasma lysate Lane 2: Mouse plasma lysate Lane 3: Rat plasma lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 21 kDa Observed band size: 21/25 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722333) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |