LHX3 Recombinant Rabbit Monoclonal Antibody [JE37-44]
cat.: HA722334
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE37-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LHX3/LIM aa 300-397. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9UBR4 Human | P50481 Mouse Entrez Gene: 170671 Rat |
| Alternative names: | CPHD 3 CPHD3 DKFZp762A2013 LHX 3 LHX3 LHX3_HUMAN LIM 3 LIM homeobox 3 LIM homeobox gene 3 LIM homeobox protein 3 LIM/homeobox protein Lhx3 LIM/homeodomain protein LHX3 Lim3 M2 LHX3 mLim-3 mLIM3 P LIM |
Images
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Fig1:
Western blot analysis of LHX3 on different lysates with Rabbit anti-LHX3 antibody (HA722334) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Neuro-2a cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722334) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.