| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE37-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SLC22A1 aa 81-180 / 554. |
| Positive control: | SW480 cell lysate, HepG2 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Basolateral cell membrane, Apical cell membrane, Lateral cell membrane, Basal cell membrane, Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O15245 Human |
| Alternative names: | hOCT1 OCT1 oct1_cds Organic cation transporter 1 S22A1_HUMAN Slc22a1 solute carrier family 22 (organic cation transporter), member 1 Solute carrier family 22 member 1 |
|
Fig1:
Western blot analysis of SLC22A1 on different lysates with Rabbit anti-SLC22A1 antibody (HA722335) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: HepG2 cell lysate Lane 3: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 70 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722335) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |