Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Monkey |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE37-67 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 61 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human SLC22A1 aa 81-180 / 554. |
Positive control: | SW480 cell lysate, HepG2 cell lysate, COS-1 cell lysate. |
Subcellular location: | Basolateral cell membrane, Apical cell membrane, Lateral cell membrane, Basal cell membrane, Cell membrane. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: O15245 Human |
Alternative names: | hOCT1 OCT1 oct1_cds Organic cation transporter 1 S22A1_HUMAN Slc22a1 solute carrier family 22 (organic cation transporter), member 1 Solute carrier family 22 member 1 |
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Fig1:
Western blot analysis of SLC22A1 on different lysates with Rabbit anti-SLC22A1 antibody (HA722335) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: HepG2 cell lysate Lane 3: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 70 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722335) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |