SLC22A1 Recombinant Rabbit Monoclonal Antibody [JE37-67]
cat.: HA722335
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Monkey
Applications: WB
Clonality: Monoclonal
Clone number: JE37-67
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 61 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SLC22A1 aa 81-180 / 554.
Positive control: SW480 cell lysate, HepG2 cell lysate, COS-1 cell lysate.
Subcellular location: Basolateral cell membrane, Apical cell membrane, Lateral cell membrane, Basal cell membrane, Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O15245 Human
Alternative names: hOCT1 OCT1 oct1_cds Organic cation transporter 1 S22A1_HUMAN Slc22a1 solute carrier family 22 (organic cation transporter), member 1 Solute carrier family 22 member 1
Images
HA722335_1.jpg Fig1: Western blot analysis of SLC22A1 on different lysates with Rabbit anti-SLC22A1 antibody (HA722335) at 1/1,000 dilution.

Lane 1: SW480 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 61 kDa
Observed band size: 70 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722335) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.