GDF3 Recombinant Rabbit Monoclonal Antibody [JE38-51]
cat.: HA722339
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41/16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GDF3 aa 315-364. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, human liver tissue lysate, mouse kidney tissue lysate, mouse liver tissue lysate, rat kidney tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Secreted, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9NR23 Human | Q07104 Mouse Entrez Gene: 500311 Rat |
| Alternative names: | C78318 ecat9 GDF 3 GDF-3 GDF3 GDF3_HUMAN Growth differentiation factor 3 Growth/differentiation factor 3 KFS3 MCOP7 MCOPCB6 MGC123990 MGC123991 RGD1564178 Vgr 2 Vgr2 |
Images
|
Fig1:
Western blot analysis of GDF3 on different lysates with Rabbit anti-GDF3 antibody (HA722339) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Human liver tissue lysate (40 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Lane 7: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/16 kDa Exposure time: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722339) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.