MSP / MST1 Recombinant Rabbit Monoclonal Antibody [JE39-26]
cat.: HA722342
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB
Clonality: Monoclonal
Clone number: JE39-26
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 80 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MST1 aa 19-68.
Positive control: HeLa cell lysate, PC-3 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, rat spleen tissue lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P26927 Human | P26928 Mouse
Entrez Gene: 24566 Rat
Alternative names: D3F15S2 DNF15S2 Hepatocyte growth factor like protein alpha chain Hepatocyte growth factor like protein Hepatocyte growth factor like protein beta chain Hepatocyte growth factor like protein homolog Hepatocyte growth factor-like protein beta chain HGFL HGFL_HUMAN Macrophage stimulating 1 (hepatocyte growth factor like) Macrophage stimulatory protein Macrophage-stimulating protein MSP MST1 NF15S2 OTTHUMP00000208927
Images
HA722342_1.jpg Fig1: Western blot analysis of MSP / MST1 on different lysates with Rabbit anti-MSP / MST1 antibody (HA722342) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: PC-3 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: COS-1 cell lysate (20 µg/Lane)
Lane 7: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 80 kDa
Observed band size: 100 kDa

Exposure time: 2 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722342) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.