| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE39-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MST1 aa 19-68. |
| Positive control: | HeLa cell lysate, PC-3 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, rat spleen tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P26927 Human | P26928 Mouse Entrez Gene: 24566 Rat |
| Alternative names: | D3F15S2 DNF15S2 Hepatocyte growth factor like protein alpha chain Hepatocyte growth factor like protein Hepatocyte growth factor like protein beta chain Hepatocyte growth factor like protein homolog Hepatocyte growth factor-like protein beta chain HGFL HGFL_HUMAN Macrophage stimulating 1 (hepatocyte growth factor like) Macrophage stimulatory protein Macrophage-stimulating protein MSP MST1 NF15S2 OTTHUMP00000208927 |
|
Fig1:
Western blot analysis of MSP / MST1 on different lysates with Rabbit anti-MSP / MST1 antibody (HA722342) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: PC-3 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Lane 7: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 80 kDa Observed band size: 100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722342) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |