ZNF217 Recombinant Rabbit Monoclonal Antibody [JE39-40]
cat.: HA722344
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE39-40
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 115 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ZNF217 aa 1000-1048.
Positive control: MCF7 cell lysate, PANC-1 cell lysate, HEK-293 cell lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O75362 Human
Alternative names: ZABC 1 ZABC1 Zinc finger protein 217 ZN 217 ZN217 ZN217_HUMAN ZNF 217 ZNF217
Images
HA722344_1.jpg Fig1: Western blot analysis of ZNF217 on different lysates with Rabbit anti-ZNF217 antibody (HA722344) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: HEK-293 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 115 kDa
Observed band size: 140 kDa

Exposure time: 1 minute 50 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722344) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.