Eph receptor B1 Recombinant Rabbit Monoclonal Antibody [JE39-46]
cat.: HA722345
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE39-46 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Eph receptor B1 aa 551-650 / 984. |
| Positive control: | U-87 MG cell lysate, COS-1 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cell membrane, Early endosome membrane, Cell projection, dendrite. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P54762 Human | Q8CBF3 Mouse | P09759 Rat |
| Alternative names: | Cek 6 EK6 ELK Elkh EPH receptor B1 Eph tyrosine kinase 2 EPH-like kinase 6 Ephb1 EPHB1_HUMAN Ephrin type B receptor 1 Ephrin type-B receptor 1 EPHT2 HEK 6 HEK6 NET Neuronally-expressed EPH-related tyrosine kinase soluble EPHB1 variant 1 Tyrosine protein kinase receptor EPH 2 Tyrosine-protein kinase receptor EPH-2 |
Images
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Fig1:
Western blot analysis of Eph receptor B1 on different lysates with Rabbit anti-Eph receptor B1 antibody (HA722345) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane) Lane 2: COS-1 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722345) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.