RAP1GAP Recombinant Rabbit Monoclonal Antibody [JE41-74]
cat.: HA722349
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE41-74
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human RAP1GAP aa 600-663.
Positive control: Mouse brain tissue lysate, rat brain tissue lysate.
Subcellular location: Golgi apparatus membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P47736 Human | A2ALS5 Mouse
Entrez Gene: 313644 Rat
Alternative names: KIAA0474 Rap1 GTPase activating protein 1 RAP1 GTPase activating protein Rap1 GTPase-activating protein 1 RAP1GA1 Rap1ga1 protein Rap1GAP Rap1GAP1 RAP1GAPII RAPGAP RPGP1_HUMAN
Images
HA722349_1.jpg Fig1: Western blot analysis of RAP1GAP on different lysates with Rabbit anti-RAP1GAP antibody (HA722349) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 73 kDa
Observed band size: 95 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722349) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.