Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE41-74 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 73 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human RAP1GAP aa 600-663. |
Positive control: | Mouse brain tissue lysate, rat brain tissue lysate. |
Subcellular location: | Golgi apparatus membrane. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P47736 Human | A2ALS5 Mouse Entrez Gene: 313644 Rat |
Alternative names: | KIAA0474 Rap1 GTPase activating protein 1 RAP1 GTPase activating protein Rap1 GTPase-activating protein 1 RAP1GA1 Rap1ga1 protein Rap1GAP Rap1GAP1 RAP1GAPII RAPGAP RPGP1_HUMAN |
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Fig1:
Western blot analysis of RAP1GAP on different lysates with Rabbit anti-RAP1GAP antibody (HA722349) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 73 kDa Observed band size: 95 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722349) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |