DCAF13 Recombinant Rabbit Monoclonal Antibody [JE46-31]
cat.: HA722352
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE46-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DCAF13 aa 1-100 / 445. |
| Positive control: | HeLa cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse colon tissue, mouse ovary tissue, rat ovary tissue. |
| Subcellular location: | Nucleus, nucleolus. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q9NV06 Human | Q6PAC3 Mouse Entrez Gene: 362902 Rat |
| Alternative names: | DCA13_HUMAN DCAF13 DDB1 and CUL4 associated factor 13 DDB1- and CUL4-associated factor 13 DKFZP564O0463 GM83 HSPC064 MGC126859 MGC138247 WD repeat and SOF domain-containing protein 1 WD repeats and SOF1 domain containing WDSOF1 |
Images
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Fig1:
Western blot analysis of DCAF13 on different lysates with Rabbit anti-DCAF13 antibody (HA722352) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Neuro-2a cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Predicted band size: 51 kDa Observed band size: 49 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722352) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-DCAF13 antibody (HA722352) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722352) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-DCAF13 antibody (HA722352) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722352) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-DCAF13 antibody (HA722352) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722352) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
DCAF13 was immunoprecipitated from 0.2 mg 293T cell lysate with HA722352 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722352 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cell lysate (input) Lane 2: HA722352 IP in 293T cell lysate Lane 3: Rabbit IgG instead of HA722352 in 293T cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.