ENDOGL1 / ENGL Recombinant Rabbit Monoclonal Antibody [JE63-12]
cat.: HA722356
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE63-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ENDOGL1 / ENGL aa 341-390 / 368. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, HUVEC cell lysate, HL-60 cell lysate, MCF7 cell lysate, human heart tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9Y2C4 Human |
| Alternative names: | Endo G like 1 Endo G-like 1 Endo/exonuclease (5'-3'), endonuclease G like ENDOGL 1 ENDOGL1 ENDOGL2 Endonuclease G like 1 Endonuclease G like 2 Endonuclease G-like 1 ENGL a ENGL ENGL B ENGLA ENGLB Exog EXOG_HUMAN MGC125944 MGC125945 mitochondrial Nuclease EXOG Nuclease EXOG mitochondrial |
Images
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Fig1:
Western blot analysis of ENDOGL1 / ENGL on different lysates with Rabbit anti-ENDOGL1 / ENGL antibody (HA722356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HL-60 cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722356) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-ENDOGL1 / ENGL antibody (HA722356) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722356) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
ENDOGL1 / ENGL was immunoprecipitated from 0.2 mg HUVEC cell lysate with HA722356 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722356 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HUVEC cell lysate (input) Lane 2: HA722356 IP in HUVEC cell lysate Lane 3: Rabbit IgG instead of HA722356 in HUVEC cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.