PEF1 Recombinant Rabbit Monoclonal Antibody [JE63-88]
cat.: HA722358
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PEF1 aa 100-200. |
| Positive control: | Jurkat cell lysate, HeLa cell lysate, 293T cell lysate, HL-60 cell lysate, rat pancreas tissue lysate. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum, Membrane, Cytoplasmic vesicle, COPII-coated vesicle membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9UBV8 Human | Q641Z8 Rat |
| Alternative names: | ABP32 PEF PEF protein with a long N terminal hydrophobic domain PEF protein with a long N-terminal hydrophobic domain pef1 PEF1_HUMAN PEF1A Peflin Penta EF hand domain containing protein 1 Penta-EF hand domain-containing protein 1 |
Images
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Fig1:
Western blot analysis of PEF1 on different lysates with Rabbit anti-PEF1 antibody (HA722358) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HL-60 cell lysate (20 µg/Lane) Lane 5: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: Lane 1-4: 20 seconds; Lane 5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722358) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.