MRPS31 Recombinant Rabbit Monoclonal Antibody [JE64-25]
cat.: HA722359
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MRPS31 aa 346-395 / 395. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, MCF7 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q92665 Human | Q61733 Mouse | B0BN56 Rat |
| Alternative names: | 28S ribosomal protein S31 28S ribosomal protein S31, mitochondrial Imogen 38 IMOGN38 mitochondrial MRP-S31 MRPS31 RT31_HUMAN S31mt |
Images
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Fig1:
Western blot analysis of MRPS31 on different lysates with Rabbit anti-MRPS31 antibody (HA722359) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: A431 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722359) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.