TAPP-1 Recombinant Rabbit Monoclonal Antibody [JE64-38]
cat.: HA722361
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE64-38 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TAPP-1 aa 300-400. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q9HB21 Human | Q8BUL6 Mouse Entrez Gene: 361659 Rat |
| Alternative names: | OTTHUMP00000020651 PH domain containing family A member 1 PH domain-containing family A member 1 PKHA1_HUMAN Pleckstrin homology domain containing A1 Pleckstrin homology domain containing family A member 1 Pleckstrin homology domain containing, family A (phosphoinositide binding specific) member Pleckstrin homology domain-containing family A member 1 PLEKHA1 Tandem PH domain containing protein 1 Tandem PH domain-containing protein 1 TAPP 1 TAPP-1 TAPP1 |
Images
|
Fig1:
Western blot analysis of TAPP-1 on different lysates with Rabbit anti-TAPP-1 antibody (HA722361) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: C6 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722361) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling TAPP-1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722361, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.