PMF-1 Recombinant Rabbit Monoclonal Antibody [JE65-48]
cat.: HA722365
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE65-48 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PMF1 aa 1-205. |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, HeLa cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HepG2, RAW264.7, C6. |
| Subcellular location: | Nucleus, Chromosome, centromere, kinetochore. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q6P1K2 Human | Q9CPV5 Mouse Entrez Gene: 681050 Rat |
| Alternative names: | NNF1R PMF 1 PMF-1 Pmf1 PMF1_HUMAN Polyamine modulated factor 1 Polyamine-modulated factor 1 |
Images
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Fig1:
Western blot analysis of PMF-1 on different lysates with Rabbit anti-PMF-1 antibody (HA722365) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722365) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling PMF-1 with Rabbit anti-PMF-1 antibody (HA722365) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMF-1 antibody (HA722365) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of RAW264.7 cells labeling PMF-1 with Rabbit anti-PMF-1 antibody (HA722365) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMF-1 antibody (HA722365) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig4:
Immunocytochemistry analysis of C6 cells labeling PMF-1 with Rabbit anti-PMF-1 antibody (HA722365) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMF-1 antibody (HA722365) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.