Human CD85d Recombinant Rabbit Monoclonal Antibody [PSH05-62]
cat.: HA722382
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD85d aa 22-460 (Extracellular). |
| Positive control: | Recombinant standard Human CD85d protein (HA210643). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-61] to Human CD85d (Capture) (HA722381) and recombinant standard Human CD85d protein (HA210643). The reference range value is 61.7-15000 pg/ml. |
| Uniprot #: | SwissProt: Q8N423 Human |
| Alternative names: | CD85 antigen-like family member D CD85d CD85d antigen ILT 4 ILT-4 ILT4 Immunoglobulin like transcript 4 Immunoglobulin-like transcript 4 Leukocyte immunoglobulin like receptor 2 Leukocyte immunoglobulin like receptor subfamily B member 2 Leukocyte immunoglobulin-like receptor 2 Leukocyte immunoglobulin-like receptor subfamily B member 2 Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2 LILRA6 LILRB2 LIR 2 LIR-2 LIR2 LIRb LIRB2_HUMAN MIR 10 MIR-10 MIR10 Monocyte/macrophage immunoglobulin like receptor 10 Monocyte/macrophage immunoglobulin-like receptor 10 |
Images
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Fig1:
Sandwich ELISA analysis of human CD85d matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722381) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Human CD85d protein (HA210643) starting from 15000 pg/ml to 0 pg/ml and detect antibody (HA722382, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD85d in human serum samples. The concentrations of CD85d were interpolated from the CD85d standard curves and corrected for sample dilution. Undiluted samples are serum 100%. The mean CD85d concentration was determined to be 4448 pg/mL in human serum. |
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Fig3:
Interpolated concentrations of spiked CD85d in human cell culture media samples. The concentrations of CD85d were measured in duplicates, interpolated from the CD85d standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.