Human GITR Recombinant Rabbit Monoclonal Antibody [PSH05-64]
cat.: HA722384
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GITR aa 26-162 (Extracellular). |
| Positive control: | Recombinant standard Human GITR protein (HA210945). |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-63] to Human GITR (Capture) (HA722383) and recombinant standard Human GITR protein (HA210945). The reference range value is 37-3000pg/ml. |
| Uniprot #: | SwissProt: Q9Y5U5 Human |
| Alternative names: | Activation inducible TNFR family receptor Activation-inducible TNFR family receptor AITR CD357 GITR GITR D GITR-D GITRD Glucocorticoid induced TNFR related protein glucocorticoid-induced tnf receptor ligand glucocorticoid-induced tnf receptors ligand Glucocorticoid-induced TNFR-related protein TNF receptor superfamily activation inducible protein TNFRSF 18 TNFRSF18 TNR18_HUMAN Tumor necrosis factor receptor superfamily member 18 Tumor necrosis factor receptor superfamily member 18 precursor |
Images
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Fig1:
Sandwich ELISA analysis of human GITR matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722383) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant human GITR protein (HA210945) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA722384, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native GITR in Jurkat and HuT-102 cell extract samples based on a 1000 µg/ml extract load. The concentrations of GITR were interpolated from the GITR standard curve and corrected for sample dilution. The mean GITR concentration was determined to be 37.69 ng/ml in HuT-102 cell extract. There was no detectable signal in Jurkat cell extract. |
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Fig3:
Interpolated concentrations of spiked GITR in human cell culture media samples. The concentrations of GITR were measured in duplicates, interpolated from the GITR standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.