Human IL-2 Receptor alpha Recombinant Rabbit Monoclonal Antibody [PSH05-65]
cat.: HA722385
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-2 Receptor alpha aa 22-240 (Extracellular). |
| Positive control: | Recombinant standard Human IL2RA protein (HA210771). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-66] to Human IL2RA (Detector) (HA722386) and recombinant standard Human IL2RA protein (HA210771). The reference range value is 8.2-2000pg/ml. |
| Uniprot #: | SwissProt: P01589 Human |
| Alternative names: | Interleukin 2 receptor alpha chain CD25 CD25 antigen IDDM10 IL 2 receptor alpha subunit IL-2 receptor subunit alpha IL-2-RA IL-2R subunit alpha IL2 RA IL2 Receptor alpha IL2-RA IL2R IL2R, alpha chain IL2RA IL2RA_HUMAN IMD41 Interleukin 2 receptor alpha Interleukin 2 receptor Interleukin-2 receptor subunit alpha p55 t-cell growth factor receptor TAC TAC antigen TCGFR |
Images
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Fig1:
Sandwich ELISA analysis of human IL2RA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722385) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human IL2RA protein (HA210771) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722386, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL2RA in human PBMC cell culture supernatant. PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 2 days. The concentrations of IL2RA measured in duplicate and interpolated from the IL2RA standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL2RA concentration was determined to be 1,306.5 pg/ml in PHA-M stimulated PBMC cell culture supernatant and 101.3 pg/ml in the unstimulated PBMC control. |
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Fig3:
Interpolated concentrations of native IL2RA in human serum samples. The concentrations of IL2RA were measured in triplicates, interpolated from the IL2RA standard curve and corrected for sample dilution. Undiluted samples are human serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean IL2RA concentration was determined to be 3,193 pg/mL in human serum. |
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Fig4:
Interpolated concentrations of spiked IL2RA in human cell culture media samples. The concentrations of IL2RA were measured in triplicates, interpolated from the IL2RA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.