Nogo Receptor Recombinant Rabbit Monoclonal Antibody [JE33-68]
cat.: HA722391
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Nogo Receptor aa 341-440 / 473. |
| Positive control: | SH-SY5Y cell lysate, HepG2 cell lysate, HEK-293 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cell membrane, Membrane raft, Cell projection, dendrite, axon, Perikaryon. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9BZR6 Human | Q99PI8 Mouse | Q99M75 Rat |
| Alternative names: | NgR Nogo 66 receptor Nogo receptor Nogor Reticulon 4 receptor Rtn4r UNQ330/PRO526 |
Images
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Fig1:
Western blot analysis of Nogo Receptor on different lysates with Rabbit anti-Nogo Receptor antibody (HA722391) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (30 µg/Lane) Lane 6: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 51 kDa Observed band size: 65 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.