Carboxypeptidase A1+A2+B Recombinant Rabbit Monoclonal Antibody [JE34-07]
cat.: HA722393
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE34-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Carboxypeptidase A1 aa 320-419 / 419. |
| Positive control: | Mouse pancreas tissue lysate, rat pancreas tissue lysate, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P15085 Human | P48052 Human | P15086 Human | Q7TPZ8 Mouse | P00731 Rat | P19222 Rat | P19223 Rat |
| Alternative names: | carboxypeptidase A1 (pancreatic) Carboxypeptidase A1 Carboxypeptidase A1 precursor CBPA1_HUMAN CPA CPA1 Pancreatic carboxypeptidase A1 Procarboxypeptidase A1 pancreatic Carboxypeptidase A2 CPA2 Carboxypeptidase B Carboxypeptidase B1 (tissue) Carboxypeptidase B1 CarboxypeptidaseB CarboxypeptidaseB1 CBPB1_HUMAN CP B1 CPB 1 CPB CPB1 DKFZp779K1333 Pancreas specific protein Pancreas-specific protein Pancreatic carboxypeptidase B Pancreatic carboxypeptidase B1 PASP PCPB Procarboxypeptidase B Procarboxypeptidase B pancreatic Protaminase Tissue carboxypeptidase B |
Images
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Fig1:
Western blot analysis of Carboxypeptidase A1+A2+B on different lysates with Rabbit anti-Carboxypeptidase A1+A2+B antibody (HA722393) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Rat pancreas tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 47 kDa Observed band size: 43 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722393) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Carboxypeptidase A1+A2+B antibody (HA722393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Carboxypeptidase A1+A2+B antibody (HA722393) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722393) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Carboxypeptidase A1+A2+B antibody (HA722393) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722393) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.