HOXA5 Recombinant Rabbit Monoclonal Antibody [JE36-98]
cat.: HA722398
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HOXA5 aa 100-200. |
| Positive control: | 293T cell lysate, SH-SY5Y cell lysate, Caco-2 cell lysate, rat heart tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P20719 Human | P52949 Rat |
| Alternative names: | Homeo box 1C Homeo box A5 Homeobox A5 Homeobox protein Hox-1C Homeobox protein Hox-A5 Homeobox protein HOXA5 HOX 1 HOX 1.3 HOX 1C HOX1 HOX1.3 HOX1C HOXA5 HXA5_HUMAN MGC9376 OTTHUMP00000217259 |
Images
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Fig1:
Western blot analysis of HOXA5 on different lysates with Rabbit anti-HOXA5 antibody (HA722398) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: Caco-2 cell lysate (20 µg/Lane) Lane 4: Rat heart tissue lysate (40 µg/Lane) Lane 5: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 45 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722398) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.