XRCC3 Recombinant Rabbit Monoclonal Antibody [JE38-43]
cat.: HA722399
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-43 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human XRCC3 aa 247-346 / 346. |
| Positive control: | NCI-H1299 cell lysate, MDA-MB-468 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, perinuclear region, Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O43542 Human | Q9CXE6 Mouse |
| Alternative names: | CMM6 DNA repair protein XRCC3 RAD51 like X ray repair complementing defective repair in Chinese hamster X ray repair complementing defective repair in Chinese hamster cells 3 X ray repair cross complementing protein 3 X-ray repair cross-complementing protein 3 XRCC 3 XRCC3 XRCC3_HUMAN |
Images
|
Fig1:
Western blot analysis of XRCC3 on different lysates with Rabbit anti-XRCC3 antibody (HA722399) at 1/1,000 dilution. Lane 1: NCI-H1299 cell lysate Lane 2: MDA-MB-468 cell lysate Lane 3: HepG2 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 45 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722399) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.