PAF1 Recombinant Rabbit Monoclonal Antibody [JE38-80]
cat.: HA722401
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PAF1 aa 1-100 / 531. |
| Positive control: | HeLa cell lysate, A549 cell lysate, RAW264.7 cell lysate, mouse colon tissue lysate, rat colon tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8N7H5 Human | Q8K2T8 Mouse | Q4V886 Rat |
| Alternative names: | F23149 1 FLJ11123 hPAF1 PAF 1 PAF1 Paf1 RNA polymerase II associated factor homolog PAF1_HUMAN Pancreatic differentiation protein 2 PD 2 PD2 PD2 protein RNA polymerase II associated factor homolog RNA polymerase II-associated factor 1 homolog |
Images
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Fig1:
Western blot analysis of PAF1 on different lysates with Rabbit anti-PAF1 antibody (HA722401) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Lane 5: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722401) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.