ADAM15 Recombinant Rabbit Monoclonal Antibody [JE39-01]
cat.: HA722402
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE39-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ADAM15 aa 250-350 (extracellular). |
| Positive control: | SW480 cell lysate, HUVEC cell lysate, A431 cell lysate, NIH/3T3 cell lysate, mouse colon tissue lysate, rat colon tissue lysate, human colon tissue, human liver tissue, human small intestine tissue. |
| Subcellular location: | Endomembrane system, Cell junction, adherens junction, Cell projection, cilium, flagellum, Cytoplasmic vesicle, secretory vesicle, acrosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q13444 Human | O88839 Mouse Entrez Gene: 57025 Rat |
| Alternative names: | A disintegrin and metalloproteinase domain 15 (metargidin) A disintegrin and metalloproteinase domain 15 ADA15_HUMAN ADAM 15 ADAM metallopeptidase domain 15 Adam15 and cysteine-rich protein 15 Disintegrin and metalloproteinase domain-containing protein 15 disintegrin-like EC 3.4.24. MDC 15 MDC-15 MDC15 Metalloprotease RGD disintegrin protein Metalloproteinase like disintegrin like and cysteine rich protein 15 Metalloproteinase-like Metargidin |
Images
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Fig1:
Western blot analysis of ADAM15 on different lysates with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution. Lane 1: SW480 cell lysate (15 µg/Lane) Lane 2: HUVEC cell lysate (15 µg/Lane) Lane 3: A431 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: Mouse colon tissue lysate (20 µg/Lane) Lane 6: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722402) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722402) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722402) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722402) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.