CSK Recombinant Rabbit Monoclonal Antibody [JE39-68]
cat.: HA722403
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE39-68
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CSK aa 50-150.
Positive control: Ramos cell lysate, Jurkat cell lysate, Raji cell lysate, HeLa cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Mouse lung tissue lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, C6 cell lysate, human tonsil tissue, mouse spleen tissue, rat spleen tissue.
Subcellular location: Cytoplasm, Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:200
Uniprot #: SwissProt: P41240 Human | P41241 Mouse | P32577 Rat
Alternative names: C SRC C SRC kinase C src Tyrosine Kinase C-SRC kinase c-src tyrosine kinase Csk A CSK CSK_HUMAN CYTOPLASMIC TYROSINE KINASE EC 2.7.10.2 MGC112926 MGC117393 MGC154049 P60 Src Protein tyrosine kinase CYL Protein-tyrosine kinase CYL Proto oncogene tyrosine protein kinase Tyrosine protein kinase CSK Tyrosine-protein kinase CSK zgc:154049
Images
HA722403_1.jpg Fig1: Western blot analysis of CSK on different lysates with Rabbit anti-CSK antibody (HA722403) at 1/1,000 dilution.

Lane 1: Ramos cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (low expression) (20 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)

Predicted band size: 51 kDa
Observed band size: 48 kDa
Exposure time: 2 minutes 22 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722403) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722403_2.jpg Fig2: Western blot analysis of CSK on different lysates with Rabbit anti-CSK antibody (HA722403) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: Raji cell lysate (20 µg/Lane)
Lane 6: Ramos cell lysate (20 µg/Lane)
Lane 7: RAW264.7 cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: Mouse spleen tissue lysate (40 µg/Lane)
Lane 10: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 51 kDa
Observed band size: 48 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722403) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722403_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CSK antibody (HA722403) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722403) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722403_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CSK antibody (HA722403) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722403) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722403_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CSK antibody (HA722403) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722403) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722403_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human heart tissue (negative) with Rabbit anti-CSK antibody (HA722403) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722403) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.