Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat, Monkey |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE40-14 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 23 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human KGF aa 1-100 / 194. |
Positive control: | HeLa cell lysate, A549 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P21781 Human | P36363 Mouse | Q02195 Rat |
Alternative names: | FGF 7 FGF-7 Fgf7 FGF7_HUMAN Fibroblast growth factor 7 HBGF 7 HBGF-7 HBGF7 Heparin binding growth factor 7 Heparin-binding growth factor 7 Keratinocyte growth factor KGF |
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Fig1:
Western blot analysis of KGF on different lysates with Rabbit anti-KGF antibody (HA722404) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 28 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722404) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |