Integrin alpha 9 Recombinant Rabbit Monoclonal Antibody [JE41-58]
cat.: HA722406
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE41-58
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 114 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Integrin alpha 9 aa 50-150.
Positive control: HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, rat liver tissue lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q13797 Human
Entrez Gene: 104099 Mouse | 685004 Rat
Alternative names: Alpha RLC Integrin alpha 4 like Integrin alpha RLC Integrin alpha-9 Integrin alpha-RLC ITA9_HUMAN ITGA 4L ITGA 9 ITGA4L ITGA9 RLC
Images
HA722406_1.jpg Fig1: Western blot analysis of Integrin alpha 9 on different lysates with Rabbit anti-Integrin alpha 9 antibody (HA722406) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 114 kDa
Observed band size: 114 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722406) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.